Fig. 3

Tissue expression of McWRKY57-like in M. canadensis and Arabidopsis thaliana. A Expression profiles of the McWRKY57-like gene in M. canadensis. Yl, young leaves; Ml, mature leaves; F, flowers; S, stems; Rh, rhizomes, Ar, adventitious roots. Total RNA was extracted from different tissues of M. canadensis plants for qRT-PCR. Values were standardized using the M. canadensis McACT gene. Values are the means of three independent experiments ± standard error (SE) (n = 3). B-H Analysis of McWRKY57-like expression in transgenic Arabidopsis plants by GUS staining. B GUS staining in flowers. Bar = 1 mm. C GUS staining in stems. Bar = 1 mm. D GUS staining in rosette leaves. Bar = 1 mm. E GUS staining in a 7-day-old seedling grown on agar. Bar = 1 mm. F GUS staining in 3-DAP siliques. DAP, day after pollination. Bar = 1 mm. G GUS staining in 10-DAP siliques. Bar = 1 mm. H. GUS staining in mature seeds. Bar = 100 μm