Fig. 7

Hieracrchiel clustering exhibiting high domain architectural similarities of identified proteins in (A) Gurez local (B) GM6, protein sequences labelled as NCBI Accession numbers. In Gurez local, Threonine dehydratase biosynthetic chloroplastic (AQK63454.1) and Translation initiation factor IF-2 (XP_008668879.1) formed a biologically meaningful group of two proteins while rest 17 proteins clustered together into another group. The later was found to form 4 clusters each containing closely related set of proteins [I: NHL domain-containing protein (AQK55089.1) & Peroxidase 2-like (XP_008670600.1)]; [II: Calcium-dependent protein kinase substrate protein (NP_001266439.2), Auxin response factor 12 (NP_001349277.1), Aquaporin PIP2-1 (ONM53258.1)]; [III: Calcium-dependent protein kinase substrate protein (NP_001266439.2), Tetratricopeptide repeat (TPR)-like superfamily protein (ONM27097.1),, F10K1.23 (AQK81609.1), Splicing factor 3B subunit 5/RDS3 complex subunit 10 (ONM04429.1), farnesylated protein 3 (AQK43300.1), rough sheath1 (ONM51210.1) & Thylakoidal processing peptidase 1 chloroplastic (AQK47553.1)] and [IV: GINS complex protein (AQK40686.1), Threonine dehydratase biosynthetic chloroplastic (AQK63454.1), RNA-metabolising metallo-beta-lactamase family protein (ONM31447.1) & IND1(iron-sulfur protein required for NADH dehydrogenase)-like (ONM07168.1)] separated at distinct nodes. In GM6, 3 groups of proteins were obtained. In the first two groups, 2 proteins each [(Flowering time control protein FCA-like (NP_001169298.1), RNA binding (ONM20750.1) & LETM1-like protein (AQK72491.1), Mitochondrial proton/calcium exchanger protein (XP_008645909.1)] were clustered together, however, the third group splitted further into another two distinct clusters of three proteins each [Aquaporin PIP2-1 (ONM53258.1), Mediator of RNA polymerase II transcription subunit 11 (AQK78980.1), Importin subunit alpha-2, partial (ONM30654.1) & Nuclear pore complex protein NUP155 (ONL95273.1), Glycosyltransferase family 28 C-terminal domain containing protein (NP_001149881.1), Nuclear pore complex protein NUP98A (ONM25687.1) based on the set of closely connected proteins