Table 2 List of the ISSR (A) and SCoT (B) primers; name, sequence, GC%, TM°C, total number of amplicons (TNAs) per primer, monomorphic amplicons (MAs), polymorphic amplicons (PAs), percentage of polymorphism (%P), polymorphic information content (PIC), resolving power (RP), and marker index (MI) as revealed by ISSR and SCoT profiles in the 12 wheat cultivars. The primer sequences were synthesized by the HVD Egypt company
(A) ISSR Primer list | |||||||||
Ser | Primer Name | Sequence (5́ › 3́) | TNAs | MAs | PAs | % P | PIC | RP | MI |
1 | ISSR-1 | AGAGAGAGAGAGAGAGYC | 13 | 7 | 6 | 46% | 0.34 | 7.69 | 0.023 |
2 | ISSR-2 | AGAGAGAGAGAGAGAGYG | 11 | 7 | 4 | 36% | 0.32 | 6.55 | 0.026 |
3 | ISSR-6 | CGCGATAGATAGATAGATA | 14 | 5 | 9 | 64% | 0.35 | 7.00 | 0.021 |
4 | ISSR-7 | GACGATAGATAGATAGATA | 12 | 5 | 7 | 58% | 0.33 | 6.67 | 0.025 |
5 | ISSR-8 | AGACAGACAGACAGACGC | 9 | 6 | 3 | 33% | 0.20 | 3.11 | 0.022 |
6 | ISSR-9 | GATAGATAGATAGATAGC | 13 | 3 | 10 | 77% | 0.32 | 5.54 | 0.022 |
7 | ISSR-12 | ACACACACACACACACYC | 8 | 7 | 1 | 13% | 0.11 | 1.50 | 0.013 |
8 | ISSR-13 | AGAGAGAGAGAGAGAGYT | 26 | 9 | 17 | 65% | 0.37 | 10.31 | 0.011 |
9 | ISSR-14 | CTCCTCCTCCTCCTCTT | 11 | 7 | 4 | 36% | 0.28 | 5.09 | 0.023 |
10 | ISSR-15 | CTCTCTCTCTCTCTCTRG | 11 | 5 | 6 | 54% | 0.26 | 4.54 | 0.023 |
11 | ISSR-19 | HVHTCCTCCTCCTCCTCC | 13 | 7 | 6 | 46% | 0.30 | 5..84 | 0.021 |
12 | ISSR-20 | HVHTGTGTGTGTGTGTGT | 10 | 7 | 3 | 30% | 0.21 | 3.40 | 0.118 |
Total | 151 | 75 | 76 | - | - | - | - | ||
Mean | 6.33 | 46.5 | 0.28 | 5.12 | 0.028 | ||||
(B) SCoT Primer list | |||||||||
Ser | Primer Name | Sequence (5́ › 3́) | TNAs | MAs | PAs | % P | PIC | RP | MI |
1 | ScoT-2 | ACCATGGCTACCACCGGC | 18 | 9 | 9 | 50% | 0.34 | 7.89 | 0.016 |
2 | SCoT-3 | ACGACATGGCGACCCACA | 16 | 7 | 9 | 56% | 0.33 | 6.75 | 0.018 |
3 | SCoT-4 | ACCATGGCTACCACCGCA | 12 | 8 | 4 | 33% | 0.19 | 3.00 | 0.015 |
4 | SCoT-5 | CAATGGCTACCACTAGCG | 27 | 5 | 22 | 81% | 0.37 | 10.00 | 0.009 |
5 | SCoT-11 | ACAATGGCTACCACTACC | 11 | 5 | 6 | 55% | 0.32 | 6.55 | 0.026 |
6 | SCoT-12 | CAACAATGGCTACCACCG | 10 | 6 | 4 | 40% | 0.32 | 6.60 | 0.028 |
7 | SCoT-13 | ACCATGGCTACCACGGCA | 15 | 6 | 9 | 60% | 0.27 | 4.93 | 0.017 |
8 | SCoT-16 | CCATGGCTACCACCGGCA | 14 | 6 | 8 | 57% | 0.32 | 6.71 | 0.021 |
9 | SCoT-20 | CAACAATGGCTACCACGC | 11 | 4 | 7 | 64% | 0.37 | 9.45 | 0.025 |
10 | SCoT-22 | CCATGGCTACCACCGCAC | 12 | 7 | 5 | 42% | 0.25 | 4.16 | 0.019 |
11 | SCoT-28 | CAACAATGGCTACCACCA | 9 | 8 | 1 | 11% | 0.17 | 2.44 | 0.018 |
Total | 155 | 71 | 84 | - | - | - | - | ||
Mean | 7.6 | 50 | 0.30 | 6.23 | 0.019 | ||||