Identification of fertility restoration candidate genes from a restorer line R186 for Gossypium harknessii cytoplasmic male sterile cotton

Table 1 Genetic analysis of Rf genes from R186 based on single gene dominant inheritance hypothesis

Population	Phenotype	O	E	O-E	(O-E)²/E	(\|O-E\|-0.5)²/E	χ²
F₂ (2020 Sanya)	Fertile	365	299	66	14.57	14.35	57.25
F₂ (2020 Sanya)	Sterile	34	100	-66	43.56	42.9	57.25
BCF₁ (2020 Sanya)	Fertile	320	243	77	24.4	24.08	47.54
BCF₁ (2020 Sanya)	Sterile	167	243	-76	23.77	23.46	47.54
F₂ (2020 Hejian)	Fertile	341	343	-2	0.01	0.01	0.02
F₂ (2020 Hejian)	Sterile	116	114	2	0.04	0.01	0.02
BCF₁ (2020 Hejian)	Fertile	212	194	18	1.67	1.58	3.16
BCF₁ (2020 Hejian)	Sterile	176	194	-18	1.67	1.58	3.16
F₂ (2021 Hejian)	Fertile	244	220	24	2.62	2.51	9.97
F₂ (2021 Hejian)	Sterile	50	74	24	7.78	7.46	9.97

Notes: O, observed value. E, expected value. df = 1, P = 0.05, χ2 = 3.84. df = 1, P = 0.01, χ2 = 6.64

ISSN: 1471-2229