Table 1 Nucleotide sequences and characteristics of primers
Gene | Encoding enzyme | Forward primer (5’ -3’) | Reverse primer (5’ -3’) | Product length (bp) |
|---|---|---|---|---|
AgMYB5 | v-myb avian myeloblastosis viral | CAAGCTCTTGGGGAACAGGT | AGGTTGGGGAGCCAGGATAA | 292 |
AgGAPDH | glyceraldehyde-3-phosphate dehydrogenase | CTCGCCCCGACATCTCTTTC | TCACTGGTCCGTGACTTTGG | 202 |
AtActin3 | β-actin | CCTTACAGAAGCACCAGCTCA | GTCCAGCAAGGTCTAAACGGA | 241 |
AtABA1 | zeaxanthin epoxidase | GCATTTCACGAGGAACCAGC | CCCATATTTGGCTGCATCGC | 230 |
AtNCED6 | nine-cis-epoxycarotenoid dioxygenase | CAGGTAACTTCGCTCCGGTT | AGTGTACCGGCAGCTGTAAC | 215 |
AtABA2 | short-chain dehydrogenase/reductase | CACCGTGCCCTGATATTCGT | GTTCACACGTATCCCGTGCT | 263 |
AtAAO3 | abscisic aldehyde oxidase | GTTATGCAGAGACCGACCCC | CTCTCGGATGTCGTGCTACC | 245 |
AtPDS3 | phytoene desaturase | CACTGTCGAAGGAGACGCTT | ATCCATTCCTCTGCTGGTGC | 297 |
AtZDS | zeta-carotene desaturase | GTGCTCCTCGTCCTGCTAAG | GTGCTGGTAGGACATCTGGG | 242 |
AtCRTISO | carotenoid isomerase | ACGTTCAAGGAGGTGGGAAC | CCCGATGAGCACACATCACT | 217 |
AtLCYB | lycopene epsilon cyclase | CGAGGAGACATGTTTGGCCT | CTGAATAGCCTGTTGCGGGA | 218 |
AtERD | early response to dehydration protein | AAGGAAAAGAGCCACCCTCG | AGTTTCTGCCAGGTAGCGTC | 214 |
AtP5CS | delta-pyrroline-5-carboxylate synthetase | CGTTGGCATGCACAGTTGAA | CCAACCTCTGCACCTAGTCC | 211 |
AtRD22 | responsive to dehydration 22 | TCAGCTCCACGACGATCCTA | GAACCAGCTTCCACCGAGAA | 204 |
AtRD29 | desiccation-responsive 29 | GAGTCACAGTTGACACGTCCT | TCCATGTTTAGTGAGACTGTTCT | 243 |