Fig. 6

A regulatory module of circRNA-miRNA-mRNA was verified by qPCR and luciferase reporter assay. a The model of circRNAs regulating mRNAs through ceRNA module to participate in nitrogen absorption of moso bamboo. b Expression analysis of PeSca_6:12,316,320|12,372,905, novel_miR156 and PH02Gene35622 in moso bamboo under nitrogen stress by qPCR. c-d Verification of the correlation between PeSca_6:12,316,320|12,372,905, novel_miR156 and PH02Gene35622 using a dual-luciferase reporter assay. NC and R-156 minmics indicated negative control and recombinant plasmid of novel_miR156, respectively. WT indicated plasmid containing wild-type sequences of mRNAs or circRNAs, and MUT indicated plasmid containing mutant type sequences of mRNAs or circRNAs, respectively. Error bars indicated SD of three technical repeats. Statistical analyses were performed by t-test, **: p < 0.01, ns: p > 0.05