Fig. 4
From: Novel R2R3 MYB transcription factors regulate anthocyanin synthesis in Aubergine tomato plants
Transactivation activity of the novel Abg R2R3 MYB proteins in tomato protoplasts. DFR promoter driving firefly luciferase gene was transactivated by effector plasmids containing: the AN2like genomic sequences from wild type (WT), Abg or Aft plants a; the MYB113 genomic sequences from WT and Abg plants and the MYB113 cds from Abg b; the THM27 genomic sequences from WT and Abg plants singularly and in combination with the AN2like genomic sequence from Abg, and the single AN2like genomic sequence from Abg c; and the AN2like cds from WT, Abg (both the short and the long transcripts) or Aft plants d. In all the transactivation assays, the effector R2R3 MYB plasmids were co-expressed with the effector plasmid containing the bHLH factor AN1. Data are expressed as relative luciferase activity (RLU) (Firefly_Luc/Renilla_Luc) with the value of the DFR promoter basal level set to 1 and are means of four biological replicates. One-way ANOVA with Tukey’s HSD post hoc test was performed. Different letters indicate significant differences at P ≤ 0.05