Table 2 Descriptions of candidate genes and their primer characteristics for RT-qPCR
Candidate protein/GenBank Accession no | Forward primer (5' to 3') | Reverse primer (5' to 3') | Amplicon size (bp) | Ta (oC) | Primer efficiency | R2 |
|---|---|---|---|---|---|---|
Uncharacterized protein LOC110648447 isoform X1 (XM_021802672.1) | CTTATCACTGAAAACAGTCGGGG | ATCCATTCCACCCATTTGCCA | 108 | 61 | 90.11 | 0.9318 |
Uncharacterized protein LOC110648447 isoform X2 (XM_021802673.1) | CGCAAAGTCGAGTATGGCCC | ACAGCTTGGAAGTTGTTGACATC | 142 | 61 | 108.25 | 0.7156 |
Uncharacterized protein LOC110648447 isoform X3 (XM_021802674.1) | ||||||
Oxygen-evolving enhancer 3–2 (XM_021783913.1) | CAATTCAGTGGCTCAACCCG | CAGGCTCAGCAGAAGAAGGT | 115 | 60 | 113.93 | 0.8514 |
17.3Â kDa class I heat shock protein-like (XM_021819868.1) | AGGCAAGCATGGAGAATGGA | TCAACCGGAGATCTCAATGGC | 92 | 60 | 114.35 | 0.8220 |
Superoxide dismutase [Cu–Zn]-like (XM_021784922.1) | AAATCACTGCCTTCCCCTGAC | ATTGTCCCGCTAACACCCTC | 161 | 59 | 115.10 | 0.9128 |
Universal stress protein PHOS34 (XM_021813028.1) | TAGCTCTGAAGTTGCGGTCC | CTGCTGCAAGGCGAGAAATC | 133 | 61 | 108.64 | 0.8771 |
Metalloendoproteinase 5-MMP-like (XM_021782202.1) | CAGTGGAAGGGCTGAGTAAGG | GAACTCCGCATCGTGGAATC | 201 | 58 | 116.46 | 0.9373 |