Table 1 Primers used in this paper
Term | Gene | Primer sequences (forward/reverse primer) |
|---|---|---|
qRT-PCR | TaMCA1 | F: GAACGTGACGGCATGGAC |
R: TGGTATGGGAGATCGAGGAC | ||
qRT-PCR | TaMCA9 | F: CCACCGACTTCAACCTCATCACG |
R: CCGATCTGCTCCTTCTCCTGGTC | ||
qRT-PCR | TaCDPK27 | F: GCCGCCTTCCAATACTTT |
R: TTATCCTGATCTACTTCGCCTA | ||
Subcellular localization | TaCDPK27 | F: AAGTCCGGAGCTAGCTCTAGAATGGGCAACGTCTGCGT |
R: GCCCTTGCTCACCATGGATCCGCTATTTGACTTTATGTTCAAG | ||
qRT-PCR | TaATG5 | F: CCAGAAAGGCCATGGAATCTAAC |
R: GCCTCTTTCAGGGAATTGTTGTA | ||
qRT-PCR | TaATG7 | F: TGACGTTATCGCTCCTGTTG |
R: ACAGCTGCTCGAGGAATAGC | ||
qRT-PCR | TaATG10 | F: TATTACTCGAGAGGAGCATCCCCAC |
R: GATTTTCAATCCTACTGCCTGACCG | ||
qRT-PCR | TaATG8 | F: GGAAAGGAGGCAAGCTGAA |
R: GCATCTCGTTAGGGACAAGGTA | ||
BMSV-VIGS | TaCDPK27 | F: CAAACATTTTTTTTTTTTTTTAGCTAGCGCCGCCTTCCAATACTTT |
R: GATTCTTCTTCCGTTGCTAGCTTATCCTGATCTACTTCGCCTA | ||
BMSV-VIGS | forward fragment of ATG7 | F: CGCGGATCCGGGAAGTTGTCTAGTGCTCG |
R: GGACTAGTCCCAAGAGTTCCAGCACCTA | ||
BMSV-VIGS | reverse fragment of ATG7 | F: ACGCGTCGACGGGAAGTTGTCTAGTGCTCG |
R: GGACTAGTCCCAAGAGTTCCAGCACCTA |