Fig. 6

Effect of silencing TaCDPK27 on autophagy activity in wheat seedling leaves infected with powdery mildew (Blumeria graminis f. sp. tritici, Bgt). (A) The effect of silencing TaCDPK27 on the relative expression analysis of TaATG5 and TaATG7 in leaves of wheat seedlings under Bgt infection. Data are shown as mean ± SD of three independent experiments. The different letters in each treatment show significant differences (P < 0.05). (B) The effect of silencing TaCDPK27 or TaATG7 on the autophagosomes formation in leaves of wheat seedings under Bgt infection which were stained with monodansylcadaverine (MDC). Fluorescence dots are autophagosomes (Bars = 10 μm). γG corresponds to BSMV-VIGS-GFP-inoculated wheat seedlings, and TaCDPK27 corresponds to BSMV-VIGS-TaCDPK27-inoculated wheat seedlings. TaATG7 corresponds to BSMV-VIGS-TaATG7-inoculated wheat seedlings