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Fig. 5 | BMC Plant Biology

Fig. 5

From: Silencing of the calcium-dependent protein kinase TaCDPK27 improves wheat resistance to powdery mildew

Fig. 5

Effect of silencing TaCDPK27 on cell death in wheat seedling leaves induced by powdery mildew (Blumeria graminis f. sp. tritici, Bgt) infection. (A) Evans blue staining showing the cell death. in leaves of wheat seedlings (Bar = 1000 μm). (B) The ratio of PCD cells and caspase-3-like activity in leaves of wheat seedlings under powdery mildew infection. (C) Transcript levels of TaMCA1 and TaMCA9 in wheat seedling leaves under powdery mildew infection. The CK corresponds to wild-type wheat seedlings, γG corresponds to BSMV-VIGS-GFP-inoculated wheat seedlings, and TaCDPK27 corresponds to BSMV-VIGS-TaCDPK27-inoculated wheat seedlings. TaATG7 corresponds to BSMV-VIGS-TaATG7-inoculated wheat seedlings. CK corresponds to wild-type wheat seedlings, γG corresponds to BSMV-VIGS-GFP-inoculated wheat seedlings, and TaCDPK27 corresponds to BSMV-VIGS-TaCDPK27-inoculated wheat seedlings. TaATG7 corresponds to BSMV-VIGS-TaATG7-inoculated wheat seedlings. Data are presented as mean ± SD from at least 3 independent experiments. The different letters in each treatment show significant differences (P < 0.05)

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BMC Plant Biology

ISSN: 1471-2229

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