Fig. 3
Effect of silencing TaCDPK27 on H2O2 and O2.− accumulation in wheat seedling leaves infected with powdery mildew (Blumeria graminis f. sp. tritici, Bgt) causal agent conidia. (A) Quantitative determination of H2O2 and O2.− accumulation in wheat seedling leaves infected with powdery mildew causal agent conidia. Data are presented as mean ± SD from at least 3 independent experiments. Bars with different letters are significantly different at P < 0.05. (B) NBT staining showing the generation and accumulation of O2−. in leaves of wheat seedlings. DAB staining showing the generation and accumulation of H2O2 in leaves of wheat seedlings. The CK corresponds to wild-type wheat seedlings, γG corresponds to BSMV-VIGS-GFP-inoculated wheat seedlings, and TaCDPK27 corresponds to BSMV-VIGS-TaCDPK27-inoculated wheat seedlings. TaATG7 corresponds to BSMV-VIGS-TaATG7-inoculated wheat seedlings. All the experiments presented here were performed at least three times, and similar results were obtained