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Fig. 5 | BMC Plant Biology

Fig. 5

From: DnaK and DnaJ proteins from Hsp70/40 family are involved in Rubisco biosynthesis in Synechocystis sp. PCC6803 and sustain the enzyme assembly in a heterologous system

Fig. 5

Rubisco and its chaperones in Synechocystis sp. PCC 6803 cells. A Soluble protein fractions from the wild-type Synechocystis, as well as its △Raf1 and △sll1384, were separated under native conditions and transferred to nitrocellulose for immune-blot analyses. The membranes were cut into four pieces and then probed with anti-RbcL, anti-DnaK, anti-DnaJ, and anti-Raf1 antibodies. The results shown here are representative, one of three independent experiments. B Comparison of RbcL amounts and Rubisco activity in protein extracts obtained from the listed Synechocystis variants. The Rubisco amount was determined by densitometry. The bars present the means of the three measurements (± SD). Statistical significance was determined using a one-way ANOVA with a Bonferroni posthoc test. *indicates the statistically significant difference (versus control) at P ≥ 0.05

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BMC Plant Biology

ISSN: 1471-2229

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