Fig. 4

Results of co-expression of LXS operon from Synechocystis sp. PCC6803 with studied chaperone proteins. A Table showing a combination of genes used for co-expression (the mixture numbering, 1–7, is consequently used in the whole figure) and post-native PAGE western blots analyzed with antibodies anti: DnaK2, RbcL, DnaJ Sll1384, RbcX, RbcS, and Raf1. The last lane in the gel is an uninduced E. coli lysate to eliminate any possible antibodies background. Native PAGE samples were loaded based on equal OD600 (optical density of an E.coli sample measured at a wavelength of 600 nm) of cells. Original (full range) blots are available in supplementary, Figure S4. B Rubisco activity was measured in bacterial lysates obtained in particular co-expression combinations. The control (dark gray) is an expression of LXS operon from Thermosynechococcus elongatus in E. coli cells (see paragraph 3.3 for the rationale for this control). Statistical significance was determined using a one-way ANOVA with a Bonferroni posthoc test. *indicates the statistically significant difference (versus control) at P ≥ 0.05. C RbcL amounts in protein extracts after E.coli co-expression. The Rubisco amount was determined by densitometry of the Anti-RbcL signal. The bars present the means of the three measurements (± SD). Lanes are named 1,2,3 which corresponds to the number of co-expression setups presented in panel A