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Fig. 3 | BMC Plant Biology

Fig. 3

From: DnaK and DnaJ proteins from Hsp70/40 family are involved in Rubisco biosynthesis in Synechocystis sp. PCC6803 and sustain the enzyme assembly in a heterologous system

Fig. 3

Effect of selected Rubisco chaperones on RbcL solubility in E. coli cells. A A table representing co-expression components and a dot blot (anti-RbcL antibodies) obtained for each (1–5) co-expression variant, indicating the presence of RbcL in a soluble fraction (S) and the total cell lysate (T). B A loading control (SDS-PAGE) to a dot-blot experiment, each line containing a volume of E. coli lysate corresponding to 20-μg total protein (T or S indicates a soluble or a total fraction). An expected migration of each factor is indicated with an arrow. The presented results are representative of three independent experiments

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