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Fig. 2 | BMC Plant Biology

Fig. 2

From: DnaK and DnaJ proteins from Hsp70/40 family are involved in Rubisco biosynthesis in Synechocystis sp. PCC6803 and sustain the enzyme assembly in a heterologous system

Fig. 2

Microscale thermophoresis (MST) determination of dissociation constants characterizing the interaction of RbcL and its chaperone(s). Points are the results of the normalized fluorescence of individual capillaries while the bold line is a fit of the 1:1 binding model. Curves (A) RbcL + DnaK + DnaJ + ATP, (B) RbcL + DnaK2 + ATP, and (C) RbcL + DnaJSll 1384 + ATP are representatives of at least three independent repetitions. Experiments run for 4 μM (A, B) or 200 nM (C) of the labeled chaperone; unfolded RbcL concentration indicated at X axis, as ligand concentration. The table at the bottom of the figure summarizes Kds being the mean of all repetitions. The orange plus sign indicates the strongest binding

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BMC Plant Biology

ISSN: 1471-2229

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