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Fig. 1 | BMC Plant Biology

Fig. 1

From: DnaK and DnaJ proteins from Hsp70/40 family are involved in Rubisco biosynthesis in Synechocystis sp. PCC6803 and sustain the enzyme assembly in a heterologous system

Fig. 1

Binding of unfolded RbcL by DnaJs (A) or DnaK (B) from Synechocystis sp. PCC 6803. Displacement of 1.8 ANS (20 mM) from hydrophobic surfaces of unfolded RbcL (25 µM) upon binding of the chaperone was measured as a decay of fluorescence intensity at 470 nm (excitation 390 nm) within a 600–800-s time scale. Arrows indicate time points of a chaperone addition (final concentration of 5 µM). Plots represent three independent measurements. ATP was added to a starting mixture (at a final concentration of 5 mM) when DnaK2 was tested

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BMC Plant Biology

ISSN: 1471-2229

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