Table 1 Primer sequences of genes encoding enzymes involved in 2-AP biosynthesis
Gene name | Accession no. | Primer sequences |
|---|---|---|
Proline dehydrogenase (PRODH) | AP014966.1 | F 5′-TCATCAGACGAGCAGAGGAGAACAGG-3’ |
|  |  | R 5′-CCCAGCATTGCAGCCTTGAACC-3’ |
Pyrroline-5-carboxylic acid synthetase2 (P5CS2) | AP014957.1 | F 5′-GAGGTTGGCATAAGCACAG-3’ |
|  |  | R 5′-CTCCCTTGTCGCCGTTC-3’ |
Ornithine aminotransferase (OAT) | AP014959.1 | F 5′-GCCCTTGGTGCTGGAGTA-3’ |
|  |  | R 5′-AGCCCTTTCAACGAGACCTT-3’ |
Diamine oxidase2 (DAO2) | AP014960.1 | F 5′-TCGTTCGCATCAAGGTTGG-3’ |
|  |  | R 5′-TCAGACAGAAGGGTGCCGTA-3’ |
Diamine oxidase4 (DAO4) | AP014960.1 | F 5′-TGGCAAGATAGAAGCAGAAGT-3’ |
|  |  | R 5′-GTCCATACGGGCAACAAA-3’ |
Betaine aldehyde dehydrogenase (BADH2) | AB09683 | F 5′-GGTTGGTCTTCCTTCAGGTGTGC-3’ |
|  |  | R 5′-CATCAACATCATCAAACACCACTAT-3’ |
OsActin | AK101613 | F 5′-CTTCATAGGAATGGAAGCTGCGGGTA-3’ |
|  |  | R 5′-CGACCACCTTGATCTTCATGCTGCTA-3’ |