Fig. 4

Expression pattern of flavonoids biosynthesis genes in wild blueberry phenotypes (V. angustifolium and V. myrtilloides) in response to Botrytis cinerea infection. A Chalcone synthase (CHS); B Flavonol synthase(FLS);C Anthocyanin reductase (ANR); D Anthocyanin synthase (ANS); E Dihydroflavonol-4-reductase (DFR). Expression of each gene is shown as a fold change in infected samples relative to their respective uninfected check/control from the same time point. Results are reported as means ± standard error of three biological replicates. Asterisks (*) indicate significant difference between infected plants and their basal expression (0 h before inoculation, hbi). Post inoculation time points (hbi/hpi) with the same letters on the horizontal bar are not significantly different from each other at α = 0.05. Broken horizontal line at onefold relative expression represents the calibrator