Fig. 4
From: Transcription elongation factor AtSPT4-2 positively modulates salt tolerance in Arabidopsis thaliana
Nuclear localization of AtSPT4-2. a Subcellular localization of the AtSPT4-2-GFP fusion protein. 35Spro:AtSPT4-2-GFP transgenic seedlings were grown on MS medium for 7 days and then transferred to liquid medium supplemented with 150 mM NaCl for 0, 15 and 30 minutes. The green fluorescence of 35Spro:AtSPT4-2-GFP transgenic root tissue was observed under a ZEISS 880 confocal laser scanning microscope with an excitation of 488 nm and an emission of 525 nm wavelengths. Bar = 20 μm. b Relative fluorescence intensity. The relative fluorescence intensity of AtSPT4-2-GFP in Fig. 4a was quantified with ImageJ software (NIH, USA). Values are the mean ± SD (n = 10 seedlings). Different letters represent significant differences between samples by one-way ANOVA (P < 0.05)