Skip to main content
Fig. 4 | BMC Plant Biology

Fig. 4

From: Betalain biosynthesis in red pulp pitaya is regulated via HuMYB132: a R-R type MYB transcription factor

Fig. 4

HuMYB132 directly binds to the promoters of HuADH1, HuCYP76AD1–1, and HuDODA1 according to Y1H and EMSA assays. A, Bait yeasts with the promoter sequences HuADH1, HuCYP76AD1–1 and HuDODA1 were cultured on synthetic dropout medium lacking Ura (SD/−Ura) supplemented with 200 ng/mL AbA concentration. B, The interaction of HuMYB132 and bait yeasts was determined by yeast growth on SD/−Leu medium with 200 ng/mL AbA. AD-prey/promoter-AbAi was used as a negative control. C-E, HuMYB132 could bind to promoters of HuADH1 (C), HuCYP76AD1–1 (D), and HuDODA1 (E) using an EMSA assay. The MRE and MBS sites are marked with red letters in the sequence of biotin and mutant probes

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us