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Fig. 3 | BMC Plant Biology

Fig. 3

From: Betalain biosynthesis in red pulp pitaya is regulated via HuMYB132: a R-R type MYB transcription factor

Fig. 3

HuMYB132 is a nucleus-localized transcription activator. A, Subcellular localization of HuMYB132 in the protoplast of N. benthamiana leaves. Bars = 20 μm. B, Diagrams of the reporter and effector vectors. C, Transcriptional activation of HuMYB132 in N. benthamiana leaves. The LUC/REN ratio of the empty BD-62SK vector was used as a calibrator (set as 1) while BD-62SK-VP16 was a positive control. Small triangles represent the distribution of data for each biological replicate. Results were expressed as means ± SD (n = 3), with significance values presented as: **** p < 0.0001. D, Transcriptional activation of HuMYB132 in yeast cells. pGBKT7 and pGBKT7–53+ pGADT7-T were used as negative and positive controls, respectively

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