Fig. 8

The in vitro Ub conjugation assay using purified BnUbc13s and AtUev1D. A Ub conjugation by BnUbc13A, B, C and AtUev1D. B Ub conjugation by BnUbc13D, E and AtUev1D. An in vitro Ub conjugation assay was performed using purified proteins as indicated. Assay samples were subjected to SDS-PAGE and western blotting analyses using an anti-Ub antibody. Free Ub and poly-Ub chains are marked. Unprocessed WB images shown in A, B are given in Supplementary Fig. S4A, B