Fig. 2

Alternative splicing and splice isoform analysis with SMRT-Seq reads. A The total number of AS events in predicted genes according to SMRT-Seq data compared with the annotated gene models. Annotation, AS events in genes expressed in gene models; SMRT-Seq, AS events in genes expressed in Iso-Seq reads. IR, intron retention; TSS, alternative transcription start site; TTS, alternative transcription termination site; ES, exon skipping; AE, alternative exon ends; and Total, all AS events. B Distribution of genes that produce one or more splice isoforms in SMRT-Seq data of G. lemaneiformis