Fig. 5

Validation of the acetylome quantified in maize with P. polysora infection. A and D Alignment of H3 (A) and H4 (D) sequences of human and maize. Asterisks (*) indicate conserved amino acid sites, dots (·) indicate relatively conserved amino acids, colons (:) indicates slightly conserved amino acids. B and E Quantification of specific Kac sites in H3 (B) and H4 (E) of proteome and acetylome. Asterisks (*) indicate fold change > 1.5. C, CML496; L, Lx9801. C Western blot showing the levels of H3 protein and acetylation levels of Lys18 (K18ac), Lys23 (K23ac) and pan-acetylation (H3AC) of H3. The relative intensities of H3 and H3 specific Kac sites at 0 h treatment was set to 1.00. F Western blot showing acetylation levels of Lys5 (K5ac), Lys8 (K8ac) and Lys12 (K12ac) of H4. The relative intensity of the H4 specific Kac sites at 0 h treatment was set to 1.00. Quant. indicates the intensity measured by ImageJ. Ratio Indicate the intensity was normalized to anti-H3