Genetic analysis of resistance to powdery mildew on 7Mg chromosome of wheat–Aegilops geniculata, development and utilization of specific molecular markers

Table 2 Expressed sequence tag-sequence-tagged site (EST–STS) and special SLAF-seq markers list 7M^g

Marker	Type	Primer (5’-3’)	Location	Geltype/Restrictionenzyme	Tm ◦C/t (h)
BE637663	EST-SSR	F: ACTGTTGCTTCGCTCCAAGT	7AL 7BL 7DL	8% non-denaturing	60
BE637663	EST-SSR	R: GTTCCATTTCCGATGTGCTC	7AL 7BL 7DL	polyacrylamide gel-	60
Marker7	SLAF-seq	F: GCTACACCGAACGACAATCA	7M^g	2% agarose gel	58
Marker7	SLAF-seq	R: AGGTGTCCGCTAAGGATTGA	7M^g	2% agarose gel	58
Marker9	SLAF-seq	F: CAGACGAGCTTGACTGCTTG	7M^g	2% agarose gel	58
Marker9	SLAF-seq	R: AATTTGTGCCGATTCAAAGG	7M^g	2% agarose gel	58
Marker65	SLAF-seq	F: GCTGCAGCAAAGCATTTACA	7M^g	2% agarose gel	58
Marker65	SLAF-seq	R: AGCACGTCCAAAAGAGGCTA	7M^g	2% agarose gel	58
Marker945	SLAF-seq	F: CTTCCGAGCTCCGGTAAGAT	7M^g	2% agarose gel	58
Marker945	SLAF-seq	R: TGATGTGGTGATCCTTTCCA	7M^g	2% agarose gel	58
Marker1661	SLAF-seq	F: TTGGCATACAAGCCAACTCA	7M^g	2% agarose gel	58
Marker1661	SLAF-seq	R: AGAGTGCTCTGGTCGGAAAG	7M^g	2% agarose gel	58
Marker2024	SLAF-seq	F: CTTTGGTCCCTTCTTTGGGT	7M^g	2% agarose gel	58
Marker2024	SLAF-seq	R: GCTTAATAGATGCCGAAGCG	7M^g	2% agarose gel	58
Marker887	SLAF-seq	F: GCATGGGTCCTGTCGACTAT	7M^g	2% agarose gel	58
Marker887	SLAF-seq	R: TCAGTGGCTGAAGGTCAGTG	7M^g	2% agarose gel	58
Marker2104	SLAF-seq	F: GCCCAAGGATGAGATGCTAA	7M^g	2% agarose gel	58
Marker2104	SLAF-seq	R: AGAAGAGGGGAAACTCGACC	7M^g	2% agarose gel	58
Marker390	SLAF-seq	F: GGAAGTTTACCGAAGATGGC	7M^g	2% agarose gel	58
Marker390	SLAF-seq	R: GGGAGCTAAAGAAAGCCGAT	7M^g	2% agarose gel	58

ISSN: 1471-2229