Fig. 5

Molecular marker development and PCR amplification in the target materials. (M) DL2000 (2 kb DNA ladder (1) CS; (2) SY159; (3) W16998; (4) YF175; (5)YF175 x NA0973-5-4-1-2-9-1 F₁ plant; (6–14) Nine genotypes in YF175 x W16998 F₂ population; (6) F₂ plant (2n = 40, missing two chromosome 7 A); (7) F₂ plant (2n = 41, adding one alien chromosome and missing two 7 A); (8) F₂ plant (2n = 41, missing one chromosome 7 A); (9) F₂ plant (2n = 42, two alien chromosome replaced two 7 A); (10) F₂ plant (2n = 42, one alien chromosome replaced one 7 A); (11) F₂ plant (2n = 42, without alien chromosome); (12) F₂ plant (2n = 43, adding two alien chromosomes and missing one chromosome 7 A); (13) F₂ plant (2n = 43, adding one alien chromosome); (14) F₂ plant (2n = 44, adding two alien chromosomes); (15) NA0973-5-4-1-2-9-1; (16)YF175 x NA0973-5-4-1-2-9-1 F₁ plant; (17–19) Three genotypes in YF175 x NA0973-5-4-1-2-9-1 F₂ population; (17) F₂ plant (2n = 42, without alien chromosome); (18) F₂ plant (2n = 43, adding one alien chromosome); (19) F₂ plant (2n = 44, adding two alien chromosomes); A-D The SLAF-seq markers amplification results with Marker9, Marker1661, Marker2024, Marker390. The red arrows indicate the 7M^g specific bands