Fig. 3
Powdery mildew reaction (A-B) and EST–STS functional molecular marker analysis of the target materials (C-D). (1) SY225. (2) CS. (3) SY159. (4) NA0973-5-4-1-2-9-1. (5) YF175. (6) YF175 × NA0973-5-4-1-2-9-1 F1 plant. (7–9) Three genotypes in YF175 NA0973-5-4-1-2-9-1 F2 population. (7) F2 plant (2n = 42, without alien chromosome). (8) F2 plant (2n = 43, adding one alien chromosome). (9) F2 plant (2n = 44, adding two alien chromosomes). (10) W16998. (11) YF175 x W16998 F1 plant. (12–20) Nine genotypes in YF175 x W16998 F2 population, (12) F2 plant (2n = 40, missing two chromosome 7 A). (13) F2 plant (2n = 41, adding one alien chromosome and missing two 7 A). (14) F2 plant (2n = 41, missing one chromosome 7 A). (15) F2 plant (2n = 42, two alien chromosomes replaced two 7 A). (16) F2 plant (2n = 42, one alien chromosome replaced one 7 A). (17) F2 plant (2n = 42, without alien chromosome). (18) F2 plant (2n = 43, adding two alien chromosomes and missing one 7 A). (19) F2 plant (2n = 43, adding one alien chromosome). (20) F2 plant (2n = 44, adding two alien chromosomes). (M) DL2000 (2 kb DNA ladder). C-D The EST–STS markers amplification results with BE637663. The red arrows state the specific bands