Fig. 2
The karyotype of the target plant was analyzed by FISH. FISH uses Oligo-pSc119.2 (green) and Oligo-pTa535 (red) as probes. The red arrow indicates the alien chromosome introduced from SY159. The white arrow indicates chromosome 1 A. The structural variation of the chromosome was indicated by the white arrow chromosome. A FISH of W16998. B FISH of F1. (C-L) F2 constructed by YF175 and W16998. C F2 plant (2n = 40, missing two chromosome 7 A). D F2 plant (2n = 41, with one alien chromosome and missing two 7 A). E F2 plant (2n = 41, missing one chromosome 7 A). F F2 plant (2n = 41, missing one chromosome 7 A). G F2 plant (2n = 42, two alien chromosomes replaced two 7 A). H F2 plant (2n = 42, one alien chromosome replaced one 7 A). I F2 plant (2n = 42, without alien chromosome). J F2 plant (2n = 43, adding two alien chromosomes and missing one 7 A). K F2 plant (2n = 43, adding one alien chromosome). L F2 plant (2n = 44, adding two alien chromosomes). Chromosomes were counterstained by DAPI (blue). The bar indicates 10 μm