Fig. 8
Protein interactions with ACRE1 in Arabidopsis thaliana. The proteins interacting with ACRE1 were isolated from T3 homozygous PmACRE1-OX transgenic lines. The vector control line was used as a control. Endogenous leaf proteins were extracted from these two lines and incubated with GFP-Trap agarose beads to identify the proteins that could interact with ACRE1. The interacting proteins that were isolated from PmACRE1-OX and the vector control were detected by western blotting with anti-GFP antibody A and Coomassie Brilliant Blue staining B