Fig. 6

Signs of O. robiniae larvae feeding in a galls. a-c Part of the cleared gall wall, stained with Trypane blue; view from the larval chamber side. a Damages of abaxial epidermis in form of craters (arrows) and surface abrasions (star) next to the vascular bundles. b Enlarged place of destroyed epidermal cells in form of crater (arrow). c Enlarged place of surface abrasion of epidermis. d, e Transverse, cryostate sections through the mature gall wall damaged by feeding of O. robiniae larvae; fresh material, stained with Ehrlich hematoxylin. Abaxial epidermis from the larval chamber side is broken and in some places absent (arrows). d The cells of nutritive tissue are optically almost empty. The groups of neighbouring cells with partly dissolved cell walls are visible (stars). e Similar image as in Fig. 6d, but here part of the gall wall with less destroyed structure. In the nutritive tissue the groups of cells with dissolved cell walls (stars), and also the intact cells with starch (s) in plastids are visible. Bar = 20 μM