Fig. 5

Mature, yellowish galls of O. robiniae with starch and lipids. a-c progressive changes in the nutritive tissue of the gall; transverse, cryostate sections stained with Ehrlich hematoksylin. Cell walls of nutritive tissue, stained on violet, are clearly bold. The cells contain both starch grains (s) and additionally liquid material (ls). a Note the sclereids belonging to the bundle sheats (white stars) around the bigger vascular bundle. b In enlarged cells of nutritive tissue (black stars), where the starch disappeares, a liquid material (ls) next to the partly dissolved the cell wall with adjacent cells (white arrows). Epidermis (eb) from the larval chamber side is damaged. c Groups of the adjacent cells with partly dissolved the cell wall (black arrow). The gall wall from the side of larval chamber (epidermis and parenchyma) is strong damaged. d, e) Cryostate sections (from the same galls as show in Fig. 5a-b) in reaction with Sudan III: cuticle on epidermis and lipids (li) in nutritive tissue are stained orange. e Lipid droplets (li) from the gall nutritive tissue with admixture of another substances are visible. f Transverse section, not dyed, through the mature gall with starch in nutritive tissue (between two epidermis) and lipids (li) in the larval chamber (free hand section, a little thick). Abbreviation: eb - abaxial epidermis, ed. - adaxial epidermis, lc - larval chamber, li - lipids, ls -liquid substances, s - starch, vb - vascular bundle. (a-f, Bar = 20 μM), (g, Bar = 50 μM)