Fig. 6

Validation of RFL_Contig3228_2154 on thirty-four genotypes using two approaches. a) Melting temperatures (Tm) from HRM analysis and b) allelic discriminations plot from rhAmp assay. Each dot represents a genotype, while the allele state (homozygous for the reference allele, homozygous for the alternate allele) are labeled with different colors. Variant 1 mean homozygous allele 1 / allele 1, whereas variant 2 refer to homozygous allele 2 / allele 2