Fig. 7
From: Developmentally regulated mitochondrial biogenesis and cell death competence in maize pollen
Developmentally regulated mitochondrial biogenesis in maize microspores and pollen. a Immunodetection of mitochondrial proteins in developing maize microspores and pollen. Proteins (ATP1, ATP2, ATP 6, ATP8, mitochondrial ATP synthase subunits 1, 2, 6 and 8, respectively; COXII, cytochrome oxidase subunit 2; NAD7, NADH dehydrogenase subunit 7; AOX, alternative oxidase) and the PORIN loading control were immunodetected following denaturing gel electrophoresis. Cropped blot images are shown boxed, and full length blots are presented in Additional File 2, Supplementary Fig. 2. Protein samples contained: Lane 1, 1 ug of protein extracted from normal (N) cytoplasm immature ear mitochondrial pellets; lanes 2–7, 10 ug of total protein extracted from N or CMS-S (S) microspore (MSP), or pollen stages: collapsed pollen (CP), young pollen (YP), starch filling pollen (SFP), mature pollen (MP). b-e Accumulation of mitochondrial proteins from CMS-S and N-cytoplasm maize microspores and developing pollen as compared to mature, N-cytoplasm pollen (set equal to 1). Error bars correspond to the standard deviation for three biological replicates. q,r,s different letters distinguish samples that differ in the relative abundance of ATP1, COXII, ATP6 or AOX at p ≤ 0.05 by a post-ANOVA Tukey’s HSD test. x,y,z different letters distinguish samples that differ in the relative abundance of ATP2, ATP8 or NAD7 at p ≤ 0.05 by the same test