Fig. 6
From: Developmentally regulated mitochondrial biogenesis and cell death competence in maize pollen
Actin filament organization and co-localization of mitochondria and actin filaments in maize pollen development. Spinning disc confocal micrographs of Phalloidin-iFluor 532 conjugate stained a-c normal (N) cytoplasm and d-e CMS-S pollen. a, d Lose actin network at the young pollen (YP) stage. b, c Organized, parallel actin arrays in N-cytoplasm starch-filling pollen (SFP) and mature pollen (MP), respectively. Parallel filaments run from the germination pore to the opposite side of the MP grain. e Disorganized, short actin filaments in CMS-S collapsed pollen (CP). f–h Spinning disc confocal micrographs of N-cytoplasm SFP. f Mitochondria-targeted green fluorescent protein (GFP) and g Phalloidin-iFluor 532 conjugate stained actin filaments (f-actin) of the same pollen grain. h Merged f and g images showing co-alignment of mitochondria with actin filaments. *, germination pore. Bars = 5 µm