Fig. 5
From: Developmentally regulated mitochondrial biogenesis and cell death competence in maize pollen
Mitochondrial morphology changes in normal (N) cytoplasm maize pollen development. a-j Spinning disc confocal micrographs of a-c young, bi-cellular pollen (YP); d-f starch filling pollen (SFP); and g-i mature pollen (MP). For each developmental stage, images of different pollen grains collected from the same anther are shown; a, d, g Bright field digital interference contrast (DIC). b, e, h, j Mitochondria-targeted green fluorescent protein (GFP). c, f, i Hoechst nuclear staining. j inset showing a transition stage between e and h. *, germination pore; arrows, Hoechst-stained nuclei. Bars = 20 µm for a, d, g; 5 µm for b, c, e, f, h, i, j