Fig. 4
From: Developmentally regulated mitochondrial biogenesis and cell death competence in maize pollen
Mitochondrial targeting of green fluorescent protein (GFP) and loss of mitochondrial membrane potential in CMS-S maize pollen development. a-l Scanning laser confocal images of a-d young, bi-cellular pollen (YP); e–h pre-collapse, starch-filling pollen (PC); and i-l collapsed pollen (CP). For each developmental stage, multiple images of the same pollen grain are shown. a, e, i Bright field digital interference contrast (DIC). b, f, j Pollen-expressed, mitochondria-targeted GFP. c, g, k Mitochondrial staining with the potentiometric dye JC-1. d, h, l Merged GFP and JC-1 images. Bars = 25 µm for a-d; 10 µm for e-l