Fig. 3
From: Developmentally regulated mitochondrial biogenesis and cell death competence in maize pollen
Mitochondrial targeting of green fluorescent protein (GFP) and mitochondrial membrane potential in normal (N) cytoplasm maize pollen development. a-l Scanning laser confocal images of a-d young, bi-cellular pollen (YP); e–h starch filling pollen (SFP); and i-l mature pollen (MP). For each developmental stage, multiple images of the same pollen grain are shown. a, e, i Bright field digital interference contrast (DIC). b, f, j Pollen-expressed, mitochondria-targeted GFP. c, g, k Mitochondrial staining with the potentiometric dye JC-1. d, h, l Merged GFP and JC-1 images. Bars = 10 µm