Fig. 2
From: Developmentally regulated mitochondrial biogenesis and cell death competence in maize pollen
Terminal dUTP Nick End Labeling (TUNEL) assay and DAPI staining of normal (N) and CMS-S pollen. a-l Tissue sections of developing maize pollen dual stained with a-f TUNEL and g-l DAPI. a, g TUNEL-negative nuclei in N-cytoplasm young pollen (YP). b, h TUNEL-positive staining in the nuclei of CMS-S YP. c, i Fully collapsed CMS-S pollen (CP) showing condensed cytoplasm with punctate, TUNEL-positive bodies. d, j Starch-filling N-cytoplasm mature pollen (MP) showing no TUNEL staining. e, k TUNEL positive staining of MP pre-treated with DNase. f, l Negative control for e, k. Arrows, TUNEL-positive nuclear regions; circles, condensed, TUNEL-positive cytosol; n, Nuclei. Bars = 50 µm