Fig. 4

Identification of imprinted lncRNAs in sunflower endosperm at 12 DAP. A Venn diagram analysis of imprinted lncRNAs. The number of imprinted lncRNAs identified in two crosses are shown in the blue (SY1/YS1) and red (SY2/YS2) circles, respectively.; B Comparison of imprinted lncRNAs in two crosses of sunflower. Non-imprinted: lncRNAs not showing significant deviation from 2:1 ratio of maternal allele to paternal allele in each reciprocal hybrid. Non-analyzed: lncRNAs without sufficient read counts. Low-stringency imprinted lncRNA: lncRNAs showing significant deviation from 2:1 ratio of maternal allele to paternal allele in each reciprocal hybrid. High-stringency imprinted lncRNAs: lncRNAs in which favorable alleles were at least five times more than those of non-favorable alleles in both directions of a reciprocal cross; C, D Two examples of imprinted lncRNAs. The expression level of transcribed regions is shown in green for SY1 and YS1; The percentages of allelic reads of two imprinted lncRNAs for specific SNP sites are shown, with red lines for the paternal allele and blue lines for the maternal allele; Black rectangle, exon; black line, intron. E-F DNA methylation level distribution in imprinted lncRNAs (E) and all lncRNAs (F) around the transcription start site (TSS) region, including CG, CHG methylation