Fig. 7

Quantitative real-time PCR validated the relative expression levels of DEGs related to 0, 2, or 4 days of CST at the transcriptional level in LJ11 and LJ25. (a) Expression levels of the twelve DEGs between LJ11 and LJ25. The line graphs indicate the log₂FPKM of DEGs derived from RNA-Seq data, while the bar graphs represent the relative expression levels of DEGs determined from qRT-PCR validation after 0, 2, or 4 days of CST. (b) Results of correlation analysis between RNA-seq and qRT-PCR. The scatterplots are comprised of red circles that were formed from the log2 fold change of RNA-seq (y-axis) and the fold change of qRT-PCR (x-axis). The R² (the correlation coefficient between qRT-PCR and RNA-seq) and the equation (the linear regression relationship) were presented. (c) The relative expression levels of DEGs in LN98325 and LJ3013 during the reproductive stage under 0, 2, or 4 days of CST. Values are presented as the means (± SD) of three biological replicates. * represent p < 0.05, ** represent p < 0.01. Error bars represent the SD