Table 2 Putative transcription start sites (TSSs) and transcription factor binding sites (TFBSs) in 2000Â bp promoter of P deficiency responsive miRNA genes in peanut
miRNA | miRNA sequence | regulation | pre-miRNA | pre-miRNA sequence | Chromosome | TSSs prediction | TFBSs prediction | TF prediction |
|---|---|---|---|---|---|---|---|---|
ahy-miR408-5p (21bp) | CUGGGAACAGG CAGAGCAUGA | down | ahy-MIR408 (122bp) | GAAGAAGAGAUGACAAAGAACUG GGAACAGGCAGAGCAUGAAUGGA ACUAUCAAUAGACACAUUUUGUUC AUUGACGCUCAUGCACUGCCUCUU CCCUGGCUCUCUCUUUCUUUUUCC UUCU | Chr06 | -1788 -1342 -1005 -700- 363 -62 | ACGT-containing element (ACE1) 1208Â ACACGTAGÂ Â Â Â 1215 W box 1145Â AATTGGTCAATATGÂ Â Â Â 1158Â | HY5 (bZIP transcription factor)Â WRKY1Â |
ahy-miR408-3p (21bp) | AUGCACUGCCU CUUCCCUGGC | down | ahy-MIR408 (122bp) | GAAGAAGAGAUGACAAAGAACUG GGAACAGGCAGAGCAUGAAUGGA ACUAUCAAUAGACACAUUUUGUUC AUUGACGCUCAUGCACUGCCUCUU CCCUGGCUCUCUCUUUCUUUUUCC UUCU | Chr06 | - | - | - |
ahy-miR398 (20bp) | UGUGUUCUCAG GUCACCCCU | down | ahy-MIR398 (153bp) | UAUCUCAGAGGAGUGAACCUGAGA ACACAAAGUAAAUUGUUUCGGAG UUUGGAAUGCCAUAAUCACAUGCA UAAUGCAUUUAUGCUUAUGCUUG UAUAUGGUACAUAUUUUCGAAUU UUAAUUUACCUUGUGUUCUCAGGU CACCCCUUUGAG | Chr19 | -1669 -1331 -798 -490 -177 | hor1 box 1861Â GTGAGTCATÂ Â Â Â 1869Â Â | BLZ1 (bZIP transcription factor)Â Â |
ahy-miR3515 (21bp) | AAUGUAGAAAA UGAACGGUAU | down | ahy-MIR3515 (174bp) | GGAGGAGAAUCAAUAAUGUUAUU UCUACAUUUCAUUUUCUAUACUCA UUCUCUGCACCUUCCUUUUAUGUA UGAUAAAGGAAAAAAUAUAAAUU UUUCUAUUAUUUUGUCAUAAAAG UGAGGUGUAGAGUAAAAAUGUAG AAAAUGAACGGUAUAAAUAACAU UGAUGUUGAGA | Chr15 | -1616 -1334 -745 -440 -127 | RY/G box 1673 CAtGTG 1678 -- 5-- 1684 CATGCA 1689 ZDRE 1756 ATGTCGACAT    1765 CG 659 aAACCGTCGATa     670 | ABI3   bZIP19; bZIP23   WLIM2 |
ahy-miR160-5p (21bp) | UGCCUGGCUCC CUGAAUGCCA | up | ahy-MIR160 (126bp) | AAAUAUUCGAAUUAUGUGUCUGCC UGGCUCCCUGAAUGCCAUGUAAGU UAGUUUGUUAAGAAAAGAUUAAC AAACGUCUUAUAUGGCAUGAAGG GAGUCACGCAGGCGAUUCAUUUUG AAACAUAU | Chr14 | -1682 -1348 -1031 -577 -181 | AG2 1710Â TcCCCAATGTcTTÂ Â Â Â 1722 L3 1495Â tAAAAGtgTAAAGAGAÂ Â Â Â 1510 | LFU;Â Dof11Â |
ahy-miR3518 (21bp) | UGACCUUUGGG GAUAUUCGUG | up | ahy-MIR3518 (160bp) | GCUAAGCGACAAUAAUUUGAGAG AUAAUAUGUAACGAGUAUCACCAA AUGUCACUCUUUUAGAUUUGUCUU AUUAGUUAAGGAGUUGCAACUUA UGAAGUAAAGGAUAAAUUAAAAG AGUGACCUUUGGGGAUAUUCGUG GCUCAUUACUUCUCAAAUUA | Chr09 | -1557 -1249 -861 -347 | ERRE 850Â AGCCGCCÂ Â Â Â Â 856 878Â AGCCGCCÂ Â Â Â Â 884 B1 1367Â GTTGACATGGÂ Â Â Â 1376 EBP BS 850Â AGCCGCCATÂ Â Â Â Â 858 878Â AGCCGCCGTÂ Â Â Â Â 886 | EREBP; RAP2.6; ERF1Â Â Opaque-2Â EBPÂ Â |