Fig. 5
MPK6 mediates the C10-HSL-induced inhibition of primary root growth downstream of ROS and upstream of NO. (a) Phosphorylation status (pMPK6) and total expression level of MPK6 in wild-type seedlings exposed to 30 µM C10-HSL with or without 500 µM CAT for 45 min. (b) Quantification of NO-specific fluorescence intensities in the roots of Col-0, rbohD/F and noa1 seedlings exposed to 30 µM C10-HSL 1 h using the NO specific florescent probe DAF-2DA. (c) Quantification of ROS-specific fluorescence intensities in the roots of Col-0, rbohD/F and noa1 seedlings exposed to 30 µM C10-HSL 15 min using the ROS specific florescent probe DCF-DA. (d) Transcriptional regulation of NOA1 gene after exposed to 30 µM C10-HSL for up to 2 day in Col-0 and rbohD/F. (e) Relative root growth of Col-0 and mpk6 seedlings exposed to 30 µM C10-HSL with or without 30 µM SNP, 1.5 mM H2O2 and 500 µM CAT for 5 d compared with untreated seedlings, n = 40. (f) Quantification of NO-specific fluorescence intensities in the roots of Col-0 and mpk6 seedlings exposed to 30 µM C10-HSL 1 h using the NO specific florescent probe DAF-2DA. (g) Quantification of ROS-specific fluorescence intensities in the roots of Col-0 and mpk6 seedlings exposed to 30 µM C10-HSL 15 min using the ROS specific florescent probe DCF-DA. Control or Con refers to solvent control. All the error bars represent +/- SD. (Different letters indicate significantly different values, P < 0.05 by Tukey’s test)