Fig. 2

NO is involved in the inhibition of primary root growth mediated by C10-HSL. (a, b) Detection of NO production in roots of Col-0 seedlings treated with C10-HSL (0–75 µM) for 0–60 min using the NO-specific fluorescent probe DAF-2DA (a) and quantification of NO-specific fluorescence intensities (b), bar = 50 μm, n = 30. (c), Root architecture of wild-type Arabidopsis exposed to 30 µM C10-HSL with or without 100 µM cPTIO, bar = 1 cm. (d) Primary root length of wild-type seedlings exposed 30 µM C10-HSL with or without 100 µM cPTIO, n = 40. (e, f) Detection of NO production in roots of Col-0 seedlings treated as described in (c) using the NO-specific fluorescent probe DAF-2DA (e) and quantification of NO-specific fluorescence intensities (f). (g), Relative primary root growth of wild-type and noa1 exposed to 30 µM C10-HSL, n = 40. For the picture c, d and g, 3-d-old Arabidopsis thaliana seedlings were transferred to half-strength MS medium supplemented with the indicated concentrations of C10-HSL or other chemicals and grown for 5 d. Control or Con refers to solvent control. All the error bars represent +/- SD. (Different letters indicate significantly different values, P < 0.05 by Tukey’s test; *P < 0.05, **P < 0.001, Student’s t test)