Fig. 4
Replacement of a DAM-specific motif alters PpeDAM6 protein interactions. Logo representation of the 15 amino acid-long motif identified by MEME as conserved across DAM-like proteins (a). Bait constructs used in yeast two-hybrid (Y2H) experiments (b) including protein PpeDAM61–191 with its natural DAM-specific motif in green (bait B1), PpeSVP1–192 (bait B2) and a chimerical construct of PpeDAM6 with the corresponding C-terminal end from PpeSVP (bait B3). Y2H analysis of protein interactions (c) between combinations of bait vectors (B1, B2 and B3) and prey vectors containing AGL42-like, MYB-like, SHP1-like, DUF1639-like, PI-like, AG-like, SEP2-like and ATP synthase-like genes. Yeast strains were grown on a minimal medium without tryptophan, leucine, histidine and adenine (left) and a chromogenic medium containing Aureobasidin A and X-α-Gal (+AbA +Gal) (right)