Table 4 Comprehensive ranking of reference genes using RefFinder
Ranking | Delta CT | BestKeeper | NormFinder | GeNorm | Comprehensive ranking |
|---|---|---|---|---|---|
1 | Actin1 | 18S rRNA | Actin1 | Actin97/GAPDH | Actin1 (2.30) |
2 | αTUB | GAPDH | αTUB | GAPDH (2.74) | |
3 | EF-1α | Actin97 | CYP | EF-1α | Actin97 (3.83) |
4 | GAPDH | βTUB | βTUB | Actin1 | EF-1α (3.87) |
5 | βTUB | EF-1α | EF-1α | UBC17 | αTUB (3.94) |
6 | UBC17 | UBC17 | UBC28 | αTUB | βTUB (4.86) |
7 | CYP | Actin1 | GAPDH | βTUB | 18S rRNA (5.18) |
8 | Actin97 | UBC28 | UBC17 | 18S rRNA | UBC17 (6.16) |
9 | 18S rRNA | CYP | Actin97 | CYP | CYP (6.42) |
10 | UBC28 | αTUB | 18S rRNA | UBC28 | UBC28 (8.32) |
11 | UBI | UBI | UBI | UBI | UBI (11.00) |
12 | UBC2 | UBC2 | UBC2 | UBC2 | UBC2 (12.00) |