De novo transcriptome assembly, gene annotation, and EST-SSR marker development of an important medicinal and edible crop, Amomum tsaoko (Zingiberaceae)

Table 6 Characterization of 18 EST-SSRs among 72 A. tsaoko accessions

Locus	Primer sequences (5′–3′)	Repeat motif	Ta (°C)	Size (bp)	NB	NPB	PPB
AM203	F: TTCAACCCCACGACACACAA	(AAGGCG)5	55	156	5	5	100.00%
	R: AGGATGAAGGCTGAGGAGGT
AM206	F: ACAGAGAACCACAGGCGAAG	(AGA)13	55	164	7	7	100.00%
	R: GACGAGTTGGGAAGGGATGG
AM207	F: GAGCCGATCGAGCTAACGAA	(AGAA)5	55	168	5	4	80.00%
	R: TGGAAAGGTAGGTGCAACGT
AM208	F: CGCACCCCAATTCTCCTTCT	(AGC)7	55	160	4	3	75.00%
	R: GCGGATCAGTATCATCCCCG
AM213	F: GTTTCCGCCGTGAACTAACG	(ATC)5	55	167	3	3	100.00%
	R: TATGGCCTAGTCACGTTGGC
AM218	F: GCTTCCTCTCCTATGACGCC	(CCGCCT)6	55	159	5	5	100.00%
	R: TGCTTTCGTCGCTTGTCTCT
AM223	F: TGTTCTTCTACTGCTGCGCT	(CCTCT)6	55	166	5	5	100.00%
	R: TGGTGTGAAGGAGAGGAGGA
AM224	F: TTGCTAGAGCTCAAGCCACC	(CGA)9	55	170	6	6	100.00%
	R: CAAAGCTCGAGGATAGGCGT
AM225	F: TCGTGATCCCTTCGCTTTGT	(CGAAGG)5	55	166	6	6	100.00%
	R: GCTCCATCGCCTCCAACATA
AM237	F: GTGTGATGGGGGTAAGGGTG	(GAA)8	55	158	4	4	100.00%
	R: AGCGCCGTAAAAGGTCCTTT
AM242	F: GAGAGCGAGTTGAGCCATGA	(GCA)12	55	166	7	7	100.00%
	R: GCCGGTGACAAAGATGGAGA
AM247	F: AAGAGAGCATCAAGAGGCCG	(GGA)10	55	163	6	6	100.00%
	R: CGACCGAACCTTGTAACCCA
AM248	F: CGTCTAGTGCTCCGGAATCC	(GGA)6	55	157	4	4	100.00%
	R: GCCATAGCTCCCTCTCCCTT
AM255	F: CGGGAACAACGACGGTAAGA	(GGCGGA)7	55	166	12	12	100.00%
	R: CGGAATCACAATCGCCATCG
AM272	F: GCGATCTCCAGGGCGAAATA	(TGC)7	55	156	4	4	100.00%
	R: TTTAGCCCTCCTCCTCCTCC
AM273	F: AAAGAAGGAATCTGGCCCGG	(TGCCTC)6	55	164	5	5	100.00%
	R: ACGGCGAAAGGTCAGCAATA
AM278	F: TGCCTGCGCTTAGTCAATCA	(TTC)7	55	157	6	6	100.00%
	R: CCCTCCAATGTTCCCAACGA
AM279	F: ATTGCAATTGAAGCAGCGCA	(TTCT)6	55	156	4	4	100.00%
	R: GGTGGTTTGCATCCATGGTG

Ta annealing temperature, NB Number of amplified bands, NPB Number of polymorphic bands; PPB Percentage of polymorphic bands

ISSN: 1471-2229