Fig. 5From: De novo transcriptome assembly, gene annotation, and EST-SSR marker development of an important medicinal and edible crop, Amomum tsaoko (Zingiberaceae)Unigene analysis of potential monoterpene metabolic pathway in A. tsaoko. Expression of candidate unigenes related to monoterpene synthesis of A. tsaoko (A). Alignment of amino acid sequences of Cluster-24,134.3 (putative 1,8-cineole synthase unigene) with other TPS proteins from Arabidopsis thaliala (AtTPS-Cin), Salvia officinalis (SoTPS-Cin), and Amomum villosum (AvTPS1-AvTPS4) using DNAMAN 8.0 (B). Phylogenetic tree constructed based on amino acid multiple sequence alignment using MEGA 5.2 with the neighbor-joining method (C), dark blue branches represent genus Amomum species (A. tsaoko and A. villosum), and red branches represent other species (A. thaliala and S. officinalis)Back to article page