Fig. 2

Effects of GsIMaT2 gene overexpression on root growth and nodule development at 10 and 20 days of rhizobia inoculation. A In vivo root length (cm), fresh root weight (gram), nodule numbers, and fresh nodule weight (gram) were examined (n = 10-12). The blue and red columns represent the effect of gene overexpression 10- and 20-days after rhizobial inoculation. Data are presented as means ± SD, and statistical significance is based on Student’s t-test (*P < 0.05; ** P < 0.01) with GUS-overexpressing hairy roots as the control. B Quantitative RT-PCR for in vivo hairy roots after 10- and 20-days from B. japonicum (USDA110) infection. The error bars indicate the SD of three qRT-PCR biological replicates. C HPLC analysis for profiling the isoflavonoids in the transgenic G. max hairy roots after 10 and 20 days post-inoculation