Skip to main content
Fig. 5 | BMC Plant Biology

Fig. 5

From: Evolution, gene expression, and protein‒protein interaction analyses identify candidate CBL-CIPK signalling networks implicated in stress responses to cold and bacterial infection in citrus

Fig. 5

Expression patterns of eight CsCBLs and 17 CsCIPKs in leaves of sweet orange trees infiltrated by water and in leaves of those infiltrated by Xcc bacteria as determined by qRT‒PCR. Two-year-old healthy and uniform bud-grafting ‘Taoye’ sweet orange trees growing in a greenhouse were subjected to the Xcc inoculation assay as described in the Methods section. Three biological replicates were used for measuring gene expression, and the experiment was repeated once with similar results. The relative expression levels of candidate genes were calculated using the 2−∆∆CT method. The CsEF1α gene was used as an internal reference for the nominalization of the transcript levels of the genes tested. Error bars indicate inferential statistics according to the SE. *indicates statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001; Student’s t test) when compared to the expression level in leaves of sweet orange trees infiltrated by water

Back to article page

BMC Plant Biology

ISSN: 1471-2229

Contact us